Rolling Circle Replication

1- Rolling circle replication's steps

In rolling circle replication the DNA is in a circular formation. Instead of both strands being a template strand, only one strand is. Nickase makes a nick in one of the strands, the outer, and this creates a 5’ phosphate and a 3’ hydroxyl. The 3’ works as a primer for the polymerase allowing it to function.  Such action will push the old “nicked” strand off of the template. When, in the long template, the sites cos are recognized a endonuclease cleaves the DNA thus ending replication.