AluI

Definition (reaction and biologic origin):

AluI is a restriction enzyme (protein that cuts DNA into fragments according to specific nucleotide sequences). It recognizes the sequence AGCT and cuts between the G and C nucleotides.

This enzyme is extracted from E. coli cells whose DNA was modified to carry the gene AluI from Arthrobacter luteus, which is a bacteria commonly found in the soil.

Uses:

   AluI is used in: molecular cloning, restriction site mapping (method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the cuts), genotyping (determines differences in genomes by comparing a DNA sequence to that of another sample or a reference sequence, and it can identifie small variations within populations), restriction fragment length polymorphism (RFLP) and single nucleotide polymorphism (SNP).

Buffer:

The buffer used in reactions with AluI is Tango buffer which is premixed with BSA (Bovin Serum Albumin, a protein derivated from cows that gives stability to the enzymes and binds with contaminants that may be presente in DNA preparations) and ensures the optimum reaction conditions for restriction enzymes.

References:

Unknown author. Date unknown. AluI. Retrieved on November 14, 2018. Available at: https://international.neb.com/products/r0137-alui#Product%20Information

Unknown author. Date unknown. AluI restriction enzyme. Retrieved on November 14, 2018. Available at: https://www.takarabio.com/products/cloning/restriction-enzymes/alui

Unknown author. Date unknown. AluI (10 U/µL). Retrieved on November 15, 2018. Available at: https://www.thermofisher.com/order/catalog/product/ER0011

Unknown author. Date unkown. What is Genotyping? Retrieved on November 15, 2018. Available at:https://www.thermofisher.com/pt/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/genotyping-analysis-real-time-pcr-information/what-is-genotyping.html

Unknown author. 2014. Restriction mapping. Retrieved on November 15, 2018. Available at: https://www.nature.com/scitable/definition/restriction-mapping-283