A
concatemer is an intermediate structure in the biosynthesis of certain DNA viruses,
consisting of a number of genomes connecter together end to end and separated
by cos site, and thought to be the intermediate precursor to the mature viral
genome. Concatemers are frequently the result of rolling circle replication,
and may be seen in the late stage of bacterial infection by phages. The term is
now extended to include any linear or circular DNA structure composed of viral
genomes joined end to end. The term is also used for circular DNAs which are
physically inseparable, one being threaded through the other.
For example
in the infection phenomenon of T-4 bacteriophages, when T-4 DNA enters a host
cell, it is first replicated as a unit, and then several genomic units are
recombined end to end to form a long DNA molecule, a concatemer. The concatemer
is then cut by endonucleases in non-specific sites, without regard to the
sequence in order to yield T4 genomes of the same size. This happens in order
to yield DNA molecules just long enough to fill a phage head.
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ReplyDeleteLigase will also work with blunt ends, although higher enzyme concentrations and different reaction conditions are required.
A 50 µL reaction containing 10,000 cpm of a radiolabeled single-stranded DNA substrate and 10 µL of enzyme solution incubated for 4 hours at 37°C resulted in less than 1.0% release of TCA-soluble counts.
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