T-4 DNA Ligase

This enzyme catalyzes the ligation of blunt and sticky ends of DNA, in only 5 minutes and PCR fragments with A overhangs, this reactions occur at the temperature of 25º. The T-4 DNA ligase catalyzes the formation of the phosphodiester bonds between the 3' hydroxyl of a chain and the 5' phophate of the other, in the presence of ATP. T-4 DNA ligase formulation was optimized so it can promote fast reaction, and a more convenient incubation. Single-strains nucleics acids aren´t substract to this enzyme.

Pedro Silva