Replicatio Factor C (RFC)

The replication factor C (Replication factor C - RFC) is a complex-key indirect involved in recruitment of the polymerase to near the replication fork and recruitment and correct positioning of complex PCNA. Composed of five subunits very conserved in eukaryotes, the complex RFC presents specific affinity for the primer-template region. The binding to the DNA molecule is ATP-dependent.

 

 

 

Fig1-Eukaryotic DNA replication: Step 1: primer synthesis by DNA polymerase - alpha (Pol-alpha); step 2: replication factor C (RFC) displacement of DNA polymerase and recruitment of proliferating cell nuclear antigen (PCNA); step 3: elongation by the newly recruited DNA polymerase-delta  holoenzyme (Pol-delta); step 4: strand displacement by Pol-delta ; step 5: cutting of the 5' displaced flap by flap endonuclease 1 (Fen1); and step 6: sealing by DNA ligase I (LigI).

 

Adriana Lima
Alexandra Meira
Bruno Oliveira