Analysis of Saccharomyces cerevisiae’s chromosome X

The final sequence of Saccaromyces cerevisiae‘s genome was assembled from roughly 300,000 independent sequence reads, with error rates from 0.5 to 1%, resulting in an estimated error rate of the final sequence of less than 3 errors in 10,000 bases (0.03%). The complete sequence was made available to the public on April 24^th, 1996.

Only 43.3 % of the yeast genes are currently classified as ‘functionally characterized’, having experimentally well-investigated properties, being members of well-defined protein families, or displaying strong homology to proteins with known biochemical functions.

The size of the genome is approximately 12071326 bp.

The chromosome X has a length of 7745442 bp, having each arm a length of 436307 bp and 309017 bp, and the total number of functional genes (ORF – Open Reading Frame) is 379. (The complete nucleotide sequence of ARS 1025 is illustrated on Figure 1).

It contains 25 Autonomously Replicating Sequences (ARS) and five of them are listed, along with their functions, on Table 1.

Table 1- Genes involved in replication

References:

·         F.Galibert, et al., 1996, Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X, EMBO J 15(9), page 1-2;

·         SGD: Saccaromyces cerevisiae Genome Snapshot – www.yeastgenome.org/genomesnapshot - October 29^th, 15h30min.

Work carried by Group 3.3: João Cruz, José Freitas, Margarida Barros and Mariana Pereira

Biochemistry 2^nd year 2018/2019 - University of Minho – October 29^th, 2018