Pancreatic RNase A

   The Pancreatic RNase A is a pyrimidine-specific endonuclease found in the pancreas of certain mammals and of some reptiles. Since this is an endonuclease that means the function of this enzyme is to degrade phosphodiester bonds.

    This enzyme is usually extracted from Bovine Pancreas, or scientists extract the enzyme using a technic called recombinant DNA. In this case, they take the gene from the pancreas of a mammal and them inserte that gene in the plasmid of an mutante strain of E. Coli.

Functions:

-  Removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification;

- RNA removal from recombinant protein preparations;

-Ribonuclease protection assays;

-Mapping single-base mutations in DNA/RNA;

- RNA sequence analysis and protection assays.

The reaction buffer is Tri-HCl, pH 7.5.

                                                  Figure 1: Structure of RNase A

References:

• ·         https://www.sciencedirect.com/topics/neuroscience/pancreatic-ribonuclease
• ·         https://www.ncbi.nlm.nih.gov/pmc/articles/PMC107705/
• ·         http://www.geneaid.com/products/enzymes/rnase-a
• ·         https://www.sigmaaldrich.com/catalog/product/roche/rnasearo?lang=pt&region=PT
• ·         https://www.sigmaaldrich.com/catalog/product/sigma/r6513?lang=pt&region=PT

      

·    Work done by Group 3.4: Henrique Sousa - A84794; Marco Malheiro - A85487; Ricardo Alves - A83636; Vítor Alves - A86253

        

     Applied Biology 2^nd year 2018/2019 - University of Minho – November 19^th, 2018