Figure 1 - A typical cosmid
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Inside the cell, the cosmid DNA is incapable of directing the synthesis of new phage particles. As an alternative the cosmid DNA replicates as a plasmid.
The length of the cloned DNA can be as long as the available space within λ cos site. A cosmid can be 8 kb or less in size, so up to 44 kb of new DNA can be inserted before the packaging limit of the λ phage particle is reached. Recombinant DNA is obtained from colonies and can be used to build genomic libraries.
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