DNA Ligase
DNA ligase is a specific type of enzyme; that catalyzes the linkage between two free ends of
double-stranded DNA chains by forming a phosphodiester bond between them, as in
the repair of damaged DNA.
There are two classes of DNA ligases:
·
The first uses NAD+
as a cofactor and only found in bacteria.
·
The second uses ATP as a
cofactor and found in eukaryotes, viruses and bacteriophages.
Biologically, DNA ligases are essential for the
joining of Okazaki fragments during replication, and for completing short-patch
DNA synthesis occurring in DNA repair process.
In addition,
DNA ligase has extensive use in molecular biology laboratories for genetic recombination experiments. Purified DNA ligase is used in gene cloning to join DNA
molecules together to form recombinant
DNA.
 |
Fig. 1: DNA ligase mechanism |
Great post. Thanks for sharing. t4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA.
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